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Products / Women's health and STI

Women's health and STI

Amur WH Vaginal Microbiota Panel A

34 vaginal microbiota targets and 3 antibiotic resistance genes.

For Research Use Only. Not for use in diagnostic procedures.

SeqOnce kit by Bandit Biosystems, Amur WH Vaginal Microbiota Panel A: carton and reagent tubes
Representative kit packaging. Tube count and labels vary by panel and format.

Overview

Qualitative multiplex detection of vaginal microbiota and selected antibiotic resistance genes. The panel covers sexually transmitted pathogens, common pathogenic yeasts, bacteria associated with vaginosis and normal Lactobacillus flora.

Targets

Sexually transmitted pathogens

  • Chlamydia trachomatis
  • Neisseria gonorrhoeae
  • Trichomonas vaginalis
  • Mycoplasma genitalium
  • Treponema pallidum
  • Haemophilus ducreyi
  • Herpes simplex virus 1 (HSV-1)
  • Herpes simplex virus 2 (HSV-2)
  • Ureaplasma urealyticum

Vaginosis-associated and other bacteria

  • Gardnerella vaginalis
  • Atopobium vaginae
  • BVAB2
  • Megasphaera 1
  • Megasphaera 2
  • Mobiluncus curtisii
  • Mobiluncus mulieris
  • Prevotella bivia
  • Mycoplasma hominis
  • Bacteroides fragilis
  • Group B Streptococcus
  • Escherichia coli
  • Enterococcus faecalis
  • Staphylococcus aureus

Lactobacillus flora

  • Lactobacillus crispatus
  • Lactobacillus gasseri
  • Lactobacillus iners
  • Lactobacillus jensenii

Yeasts

  • Candida albicans
  • Candida auris
  • Candida glabrata
  • Candida krusei
  • Candida lusitaniae
  • Candida parapsilosis
  • Candida tropicalis

Resistance genes

  • mecA (methicillin)
  • vanA (vancomycin)
  • vanB (vancomycin)

Internal control

  • Human RNase P (hRPP)

Specifications

FormatqPCR, qualitative multiplex
Primer and probe mix10 tubes
Kit size48 reactions of each of the 10 primer and probe mixes
Larger kits500 to 1,000 reaction kits are available as a custom order. Request a quote.
Positive controls4 tubes, included
FluorophoresFAM, HEX, Cy5, ROX
Sample inputDNA extracted from urine or swab samples using a method that recovers bacterial, viral and fungal DNA. Performance to be verified by the user.
Reaction volume15 µL (11 µL reaction mix + 4 µL sample), in 96- or 384-well plates
Run timeLess than 60 minutes
Recommended master mixSeqOnce™ qPCR Multiplex Amplification Kit 3600, PN 500024. Sold separately.
Compatible instrumentsInstruments that detect FAM, HEX, Cy5, ROX (or equivalent wavelengths), including:
Bio-Rad CFX96 / CFX384 and CFX Opus
Roche LightCycler 480
Applied Biosystems QuantStudio 3, 5, 6, 7 and 12, and 7500 series
Storage−20 °C
Shelf lifeUp to 24 months from the date of manufacture, depending on lot and components

Recommended cycling

  • Enzyme activation: 95 °C for 2 minutes
  • 40 cycles: 95 °C for 3 seconds, then 59 °C for 30 seconds
  • Collect fluorescence during the 59 °C step on FAM, HEX, Cy5 and ROX (or equivalent channels)
  • Prepare each tube's reaction mix separately; do not combine primer and probe mixes

Quality

  • Lot tested. Every lot is tested before release.
  • Safety Data Sheet. Available on request from info@bandit.bio.
  • Stability. Up to 24 months from the date of manufacture when stored as directed. Varies by lot and components.
  • Made in the USA. Manufactured in Carlsbad, California and Grand Rapids, Michigan.

Ordering and pricing

OptionSKUReactionsPriceOrder
Complete kit
Panel and master mix500001 + 50002448
À la carte
Panel only: Amur WH Vaginal Microbiota Panel A50000148
Master mix only: SeqOnce™ qPCR Multiplex Amplification Kit 360050002448

Prices in U.S. dollars, excluding tax and shipping. Sales tax is calculated at checkout. Volume and custom pricing on request.