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BanditBiosystems

Products / Veterinary

Veterinary

Canine Enteric Pathogen qPCR Panel

13 bacterial, parasitic and viral targets associated with canine enteric infection and diarrhea.

For Research Use Only. Not for use in diagnostic procedures.

SeqOnce kit by Bandit Biosystems, Canine Enteric Pathogen qPCR Panel: carton and reagent tubes
Representative kit packaging. Tube count and labels vary by panel and format.

Overview

Qualitative multiplex RT-qPCR detection of 13 canine enteric targets across bacteria, parasites and viruses in four tubes. When paired with appropriate sampling and extraction methods, the panel is suitable for specimens from veterinary sources. The SPRY1 multi-species internal control monitors extraction and PCR inhibition.

Targets

Bacteria

  • Clostridium difficile toxin A/B
  • Clostridium perfringens enterotoxin (CPE)
  • Clostridium perfringens alpha toxin (CPA)
  • Clostridium perfringens NetE/NetF
  • Salmonella spp.
  • Campylobacter (jejuni and coli)
  • Lawsonia intracellularis

Parasites

  • Cryptosporidium spp.
  • Giardia spp.

Viruses

  • Canine circovirus
  • Canine distemper virus
  • Canine enteric coronavirus
  • Canine parvovirus

Internal control

  • SPRY1 multi-species control

Specifications

FormatRT-qPCR, qualitative multiplex
Primer and probe mix4 tubes
Kit size100 reactions of each of the 4 primer and probe mixes
Larger kits500 to 1,000 reaction kits are available as a custom order. Request a quote.
Positive controlsVeterinary Enteric Pathogen Positive Control, mixed (PN 450051) or three individual tubes (PN 450055, 450056, 450057), included
FluorophoresFAM, HEX, Cy5, ROX
Sample inputExtracted DNA and RNA from specimens from veterinary sources, using a validated extraction method. Performance to be verified by the user.
Reaction volume15 µL (11 µL reaction mix + 4 µL sample), in 96- or 384-well plates
Recommended master mixBandit Biosystems RT-qPCR Multiplex Amplification Kit 3000, PN 500055. Sold separately.
Compatible instrumentsInstruments that detect FAM, HEX, Cy5, ROX (or equivalent wavelengths), including:
Bio-Rad CFX96 / CFX384 and CFX Opus
Roche LightCycler 480
Applied Biosystems QuantStudio 3, 5, 6, 7 and 12, and 7500 series
Storage−20 °C
Shelf lifeUp to 24 months from the date of manufacture, depending on lot and components

Tube and dye layout

TubeFAMHEXCy5ROX
Tube 1C. difficile toxin A/BC. perfringens CPEC. perfringens CPAC. perfringens NetE/NetF
Tube 2SPRY1 controlCanine circovirusCanine distemper virusCanine enteric coronavirus
Tube 3SPRY1 controlSalmonella spp.Cryptosporidium spp.Campylobacter (jejuni and coli)
Tube 4SPRY1 controlGiardia spp.Canine parvovirusLawsonia intracellularis

HEX may be replaced by VIC or JOE if your instrument does not have a HEX channel.

Recommended cycling

  • Reverse transcription: 50 °C for 15 minutes
  • Enzyme activation: 95 °C for 2 minutes
  • 40 cycles: 95 °C for 3 seconds, then 59 °C for 30 seconds
  • Collect fluorescence during the 59 °C step

Quality

  • Lot tested. Every lot is tested before release.
  • Safety Data Sheet. Available on request from info@bandit.bio.
  • Stability. Up to 24 months from the date of manufacture when stored as directed. Varies by lot and components.
  • Made in the USA. Manufactured in Carlsbad, California and Grand Rapids, Michigan.

Ordering and pricing

OptionSKUReactionsPriceOrder
Canine Enteric Pathogen qPCR Panel500053100On requestQuote
Bandit Biosystems RT-qPCR Multiplex Amplification Kit 3000500055100On requestQuote
Panel and master mix bundle500053 + 500055100On requestQuote

Prices in U.S. dollars, excluding tax and shipping. Sales tax is calculated at checkout. Volume and custom pricing on request.