Veterinary
Feline Enteric Pathogen qPCR Panel
11 bacterial, parasitic and viral targets associated with feline enteric infection and diarrhea.
For Research Use Only. Not for use in diagnostic procedures.
Overview
Qualitative multiplex RT-qPCR detection of 11 feline enteric targets across bacteria, parasites and viruses in three tubes. When paired with appropriate sampling and extraction methods, the panel is suitable for specimens from veterinary sources. The SPRY1 multi-species internal control monitors extraction and PCR inhibition.
Targets
Bacteria
Parasites
Viruses
Internal control
Specifications
| Format | RT-qPCR, qualitative multiplex |
|---|---|
| Primer and probe mix | 3 tubes |
| Kit size | 100 reactions of each of the 3 primer and probe mixes |
| Larger kits | 500 to 1,000 reaction kits are available as a custom order. Request a quote. |
| Positive controls | Veterinary Enteric Pathogen Positive Control, mixed (PN 450051) or individual tubes (PN 450055, 450056, 450057), included |
| Fluorophores | FAM, HEX, Cy5, ROX |
| Sample input | Extracted DNA and RNA from specimens from veterinary sources, using a validated extraction method. Performance to be verified by the user. |
| Reaction volume | 15 µL (11 µL reaction mix + 4 µL sample), in 96- or 384-well plates |
| Recommended master mix | Bandit Biosystems RT-qPCR Multiplex Amplification Kit 2250, PN 500044. Sold separately. |
| Compatible instruments | Instruments that detect FAM, HEX, Cy5, ROX (or equivalent wavelengths), including: Bio-Rad CFX96 / CFX384 and CFX Opus Roche LightCycler 480 Applied Biosystems QuantStudio 3, 5, 6, 7 and 12, and 7500 series |
| Storage | −20 °C |
| Shelf life | Up to 24 months from the date of manufacture, depending on lot and components |
Tube and dye layout
| Tube | FAM | HEX | Cy5 | ROX |
|---|---|---|---|---|
| Tube 1 | C. difficile toxin A/B | C. perfringens CPE | C. perfringens CPA | Feline parvovirus 2 |
| Tube 2 | SPRY1 control | Salmonella spp. | Cryptosporidium spp. | Campylobacter (jejuni and coli) |
| Tube 3 | Feline enteric coronavirus | Giardia spp. | Tritrichomonas foetus / T. blagburni | Toxoplasma gondii |
HEX may be replaced by VIC or JOE if your instrument does not have a HEX channel.
Recommended cycling
- Reverse transcription: 50 °C for 15 minutes
- Enzyme activation: 95 °C for 2 minutes
- 40 cycles: 95 °C for 3 seconds, then 59 °C for 30 seconds
- Collect fluorescence during the 59 °C step
Quality
- Lot tested. Every lot is tested before release.
- Safety Data Sheet. Available on request from info@bandit.bio.
- Stability. Up to 24 months from the date of manufacture when stored as directed. Varies by lot and components.
- Made in the USA. Manufactured in Carlsbad, California and Grand Rapids, Michigan.
Ordering and pricing
| Option | SKU | Reactions | Price | Order |
|---|---|---|---|---|
| Feline Enteric Pathogen qPCR Panel | 500054 | 100 | On request | Quote |
| Bandit Biosystems RT-qPCR Multiplex Amplification Kit 2250 | 500044 | 100 | On request | Quote |
| Panel and master mix bundle | 500054 + 500044 | 100 | On request | Quote |
Prices in U.S. dollars, excluding tax and shipping. Sales tax is calculated at checkout. Volume and custom pricing on request.