Veterinary
Feline Respiratory XL qPCR Panel
6 bacterial and viral targets associated with feline respiratory infection.
For Research Use Only. Not for use in diagnostic procedures.
Overview
Qualitative multiplex RT-qPCR detection of six feline respiratory targets in two tubes. When paired with appropriate sampling and extraction methods, the panel is suitable for specimens from veterinary sources. The SPRY1 multi-species internal control monitors extraction and PCR inhibition.
Targets
Bacteria
Viruses
Internal control
Specifications
| Format | RT-qPCR, qualitative multiplex |
|---|---|
| Primer and probe mix | 2 tubes |
| Kit size | 100 reactions of each of the 2 primer and probe mixes |
| Larger kits | 500 to 1,000 reaction kits are available as a custom order. Request a quote. |
| Positive controls | Feline Respiratory XL Positive Control, combined (PN 450044) or individual RNA and DNA tubes (PN 450058, 450060), included |
| Fluorophores | FAM, HEX, Cy5, ROX |
| Sample input | Extracted DNA and RNA from specimens from veterinary sources, using a validated extraction method. Performance to be verified by the user. |
| Reaction volume | 15 µL (11 µL reaction mix + 4 µL sample), in 96- or 384-well plates |
| Recommended master mix | Bandit Biosystems RT-qPCR Multiplex Amplification Kit 1500, PN 500046. Sold separately. |
| Compatible instruments | Instruments that detect FAM, HEX, Cy5, ROX (or equivalent wavelengths), including: Bio-Rad CFX96 / CFX384 and CFX Opus Roche LightCycler 480 Applied Biosystems QuantStudio 3, 5, 6, 7 and 12, and 7500 series |
| Storage | −20 °C |
| Shelf life | Up to 24 months from the date of manufacture, depending on lot and components |
Tube and dye layout
| Tube | FAM | HEX | Cy5 | ROX |
|---|---|---|---|---|
| Tube 1 | SPRY1 control | Feline calicivirus | Influenza A (all strains) | — |
| Tube 2 | Chlamydophila felis | Bordetella bronchiseptica | Mycoplasma felis | Feline herpesvirus 1 |
HEX may be replaced by VIC or JOE if your instrument does not have a HEX channel.
Recommended cycling
- Reverse transcription: 50 °C for 15 minutes
- Enzyme activation: 95 °C for 2 minutes
- 40 cycles: 95 °C for 3 seconds, then 59 °C for 30 seconds
- Collect fluorescence during the 59 °C step
Quality
- Lot tested. Every lot is tested before release.
- Safety Data Sheet. Available on request from info@bandit.bio.
- Stability. Up to 24 months from the date of manufacture when stored as directed. Varies by lot and components.
- Made in the USA. Manufactured in Carlsbad, California and Grand Rapids, Michigan.
Ordering and pricing
| Option | SKU | Reactions | Price | Order |
|---|---|---|---|---|
| Feline Respiratory XL qPCR Panel | 500045 | 100 | On request | Quote |
| Bandit Biosystems RT-qPCR Multiplex Amplification Kit 1500 | 500046 | 100 | On request | Quote |
| Panel and master mix bundle | 500045 + 500046 | 100 | On request | Quote |
Prices in U.S. dollars, excluding tax and shipping. Sales tax is calculated at checkout. Volume and custom pricing on request.