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Products / Urinary tract

Urinary tract

Lioness UT Microbiota Panel A

20 bacterial species and 4 yeasts common in the urinary tract.

For Research Use Only. Not for use in diagnostic procedures.

SeqOnce kit by Bandit Biosystems, Lioness UT Microbiota Panel A: carton and reagent tubes
Representative kit packaging. Tube count and labels vary by panel and format.

Overview

Qualitative multiplex detection of the 20 most common bacterial species and 4 yeast species associated with urinary tract infection. Run it with the Pongo Antibiotic Resistance Panel A on the same extract for a combined pathogen and resistance profile.

Targets

Bacteria

  • Escherichia coli
  • Klebsiella pneumoniae
  • Klebsiella oxytoca
  • Klebsiella aerogenes
  • Proteus mirabilis
  • Proteus vulgaris
  • Pseudomonas aeruginosa
  • Acinetobacter baumannii
  • Enterobacter cloacae
  • Citrobacter freundii
  • Morganella morganii
  • Providencia stuartii
  • Serratia marcescens
  • Enterococcus faecalis
  • Enterococcus faecium
  • Staphylococcus aureus
  • Staphylococcus saprophyticus
  • Streptococcus agalactiae (Group B)
  • Mycoplasma hominis
  • Ureaplasma urealyticum

Yeasts

  • Candida albicans
  • Candida glabrata
  • Candida parapsilosis
  • Candida tropicalis

Internal control

  • Human RNase P (hRPP)

Specifications

FormatqPCR, qualitative multiplex
Primer and probe mix8 tubes
Kit size48 reactions of each of the 8 primer and probe mixes
Larger kits500 to 1,000 reaction kits are available as a custom order. Request a quote.
Kit formatsStandard primer and probe mix (add master mix), or Ready-2-Test pre-mix
Positive controls3 tubes, included
FluorophoresFAM, HEX, Cy5, ROX
Sample inputDNA extracted from urine or swab samples using a method that recovers bacterial, viral and fungal DNA. Performance to be verified by the user.
Reaction volume15 µL (11 µL reaction mix + 4 µL sample), in 96- or 384-well plates
Run timeApproximately 60 minutes
Recommended master mixSeqOnce™ qPCR Multiplex Amplification Kit 3000, PN 500023. Sold separately.
Compatible instrumentsInstruments that detect FAM, HEX, Cy5, ROX (or equivalent wavelengths), including:
Bio-Rad CFX96 / CFX384 and CFX Opus
Roche LightCycler 480
Applied Biosystems QuantStudio 3, 5, 6, 7 and 12, and 7500 series
Storage−20 °C
Shelf lifeUp to 24 months from the date of manufacture, depending on lot and components

Recommended cycling

  • Enzyme activation: 95 °C for 2 minutes
  • 40 cycles: 95 °C for 3 seconds, then 59 °C for 30 seconds
  • Collect fluorescence during the 59 °C step on FAM, HEX, Cy5 and ROX (or equivalent channels)
  • Prepare each tube's reaction mix separately; do not combine primer and probe mixes

Quality

  • Lot tested. Every lot is tested before release.
  • Safety Data Sheet. Available on request from info@bandit.bio.
  • Stability. Up to 24 months from the date of manufacture when stored as directed. Varies by lot and components.
  • Made in the USA. Manufactured in Carlsbad, California and Grand Rapids, Michigan.

Ordering and pricing

OptionSKUReactionsPriceOrder
Complete kit
Panel and master mix500012 + 50002348
À la carte
Panel only: Lioness UT Microbiota Panel A50001248
Master mix only: SeqOnce™ qPCR Multiplex Amplification Kit 300050002348

Prices in U.S. dollars, excluding tax and shipping. Sales tax is calculated at checkout. Volume and custom pricing on request.

Runs well with

Lioness+ UTI/ABR Combo Panel (save 10%)View →Pongo Antibiotic Resistance Panel AView →