Products / Wound and antibiotic resistance
Wound and antibiotic resistance
Pongo Antibiotic Resistance Panel A
24 antibiotic resistance genes, run on the same extract as your pathogen panel.
For Research Use Only. Not for use in diagnostic procedures.
Overview
Qualitative multiplex detection of antibiotic resistance genes found in bacteria. It is designed to run on the same extracted sample as the Glyphis Wound and Lioness UT panels, so labs get pathogen and resistance results from one extraction.
Targets
Beta-lactamases and carbapenemases
Other resistance genes
Specifications
| Format | qPCR, qualitative multiplex |
|---|---|
| Primer and probe mix | 6 tubes |
| Kit size | 48 reactions of each of the 6 primer and probe mixes |
| Larger kits | 500 to 1,000 reaction kits are available as a custom order. Request a quote. |
| Kit formats | Standard primer and probe mix (add master mix), or Ready-2-Test pre-mix |
| Positive controls | 3 tubes, included |
| Fluorophores | FAM, HEX, Cy5, ROX |
| Sample input | DNA extracted from bacterial samples. Performance to be verified by the user. |
| Reaction volume | 15 µL (11 µL reaction mix + 4 µL sample), in 96- or 384-well plates |
| Run time | Approximately 60 minutes |
| Recommended master mix | SeqOnce™ qPCR Multiplex Amplification Kit 2400, PN 500022. Sold separately. |
| Compatible instruments | Instruments that detect FAM, HEX, Cy5, ROX (or equivalent wavelengths), including: Bio-Rad CFX96 / CFX384 and CFX Opus Roche LightCycler 480 Applied Biosystems QuantStudio 3, 5, 6, 7 and 12, and 7500 series |
| Storage | −20 °C |
| Shelf life | Up to 24 months from the date of manufacture, depending on lot and components |
Recommended cycling
- Enzyme activation: 95 °C for 2 minutes
- 40 cycles: 95 °C for 3 seconds, then 59 °C for 30 seconds
- Collect fluorescence during the 59 °C step on FAM, HEX, Cy5 and ROX (or equivalent channels)
- Prepare each tube's reaction mix separately; do not combine primer and probe mixes
Quality
- Lot tested. Every lot is tested before release.
- Safety Data Sheet. Available on request from info@bandit.bio.
- Stability. Up to 24 months from the date of manufacture when stored as directed. Varies by lot and components.
- Made in the USA. Manufactured in Carlsbad, California and Grand Rapids, Michigan.
Ordering and pricing
| Option | SKU | Reactions | Price | Order |
|---|---|---|---|---|
| Complete kit | ||||
| Panel and master mix | 500003 + 500022 | 48 | Sign in | |
| À la carte | ||||
| Panel only: Pongo Antibiotic Resistance Panel A | 500003 | 48 | Sign in | |
| Master mix only: SeqOnce™ qPCR Multiplex Amplification Kit 2400 | 500022 | 48 | Sign in | |
Prices in U.S. dollars, excluding tax and shipping. Sales tax is calculated at checkout. Volume and custom pricing on request.