Veterinary
Ringworm qPCR Panel
Microsporum and Trichophyton, the dermatophyte fungi associated with ringworm, in a single tube.
For Research Use Only. Not for use in diagnostic procedures.
Overview
Qualitative single-tube multiplex qPCR detection of Microsporum spp. (including M. canis, M. ferrugineum and M. audouinii) and Trichophyton spp. (including T. mentagrophytes, T. tonsurans and T. equinum). When paired with appropriate sampling and extraction methods, the panel is suitable for specimens from veterinary sources. The SPRY1 multi-species internal control monitors extraction and PCR inhibition.
Targets
Fungi
Internal control
Specifications
| Format | qPCR, qualitative multiplex |
|---|---|
| Primer and probe mix | 1 tube |
| Kit size | 100 reactions |
| Larger kits | 500 to 1,000 reaction kits are available as a custom order. Request a quote. |
| Positive controls | Ringworm Positive Control (PN 450042), included |
| Fluorophores | FAM, HEX, Cy5 |
| Sample input | Extracted DNA from specimens from veterinary sources, using a validated extraction method. Performance to be verified by the user. |
| Reaction volume | 15 µL (11 µL reaction mix + 4 µL sample), in 96- or 384-well plates |
| Recommended master mix | Bandit Biosystems qPCR Multiplex Amplification Kit 750, PN 500042. Sold separately. |
| Compatible instruments | Instruments that detect FAM, HEX, Cy5 (or equivalent wavelengths), including: Bio-Rad CFX96 / CFX384 and CFX Opus Roche LightCycler 480 Applied Biosystems QuantStudio 3, 5, 6, 7 and 12, and 7500 series |
| Storage | −20 °C |
| Shelf life | Up to 24 months from the date of manufacture, depending on lot and components |
Tube and dye layout
| Tube | FAM | HEX | Cy5 |
|---|---|---|---|
| Tube 1 | SPRY1 control | Microsporum spp. | Trichophyton spp. |
HEX may be replaced by VIC or JOE if your instrument does not have a HEX channel.
Recommended cycling
- Enzyme activation: 95 °C for 2 minutes
- 40 cycles: 95 °C for 3 seconds, then 59 °C for 30 seconds
- Collect fluorescence during the 59 °C step
Quality
- Lot tested. Every lot is tested before release.
- Safety Data Sheet. Available on request from info@bandit.bio.
- Stability. Up to 24 months from the date of manufacture when stored as directed. Varies by lot and components.
- Made in the USA. Manufactured in Carlsbad, California and Grand Rapids, Michigan.
Ordering and pricing
| Option | SKU | Reactions | Price | Order |
|---|---|---|---|---|
| Ringworm qPCR Panel | 500041 | 100 | On request | Quote |
| Bandit Biosystems qPCR Multiplex Amplification Kit 750 | 500042 | 100 | On request | Quote |
| Panel and master mix bundle | 500041 + 500042 | 100 | On request | Quote |
Prices in U.S. dollars, excluding tax and shipping. Sales tax is calculated at checkout. Volume and custom pricing on request.